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Endogenous T is shown by immunofluorescence using the V22 antibody. Quantification of the number of cells showing endogenous T in the nucleus is shown on the right side of the panel (mean n=3 independent experiments, where N=200 cells where counted from each group, ANOVA). Scale bar 20 (d) Cell lysates of PC 3U [url=http://www.rtnagel.com/louboutinpascher.php]louboutin[/url] cells treated with TGF in the presence or absence of TAPI 2 as indicated, were fractionated into cytoplasmic and nuclear proteins and subjected to SDS gel electrophoresis, followed by immunoblotting using V22 antibody, which recognizes both the T full length (T and intracellular domain of T (T and lamin A served as controls for the cytoplasmic and nuclear fractions, respectively.
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These selection rates reflect the likelihood that a termination event will occur before selenocysteine incorporation when the rsUGA is at the A site during a given ribosomal passage. For example, a decrease in termination value observed with the weak stop signal ('W') (Figure 1B) reflected a 2 fold decrease in the rate of productive RF selection into the A site (Figure 1C), allowing for a relative increase in the competitiveness of Sec tRNASec and increasing the likelihood that it would be the successful decoding molecule. In contrast, the two stronger stop signals have a 1.3 to 1.4 fold increase in RRF, decreasing the competitiveness of the Sec tRNASec.
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